This three-day informal global dialogue on Digital Sequence Information (DSI) brought together some 40 representatives from governments, indigenous peoples and local communities (IPLCs), the private sector, academia, and international organisations to advance the operationalisation of the multilateral mechanism for benefit-sharing from the use of DSI (MLM), including the Cali Fund, established under CBD COP Decision 16/2. Key Messages emerging from the meeting included the importance of: - Moving from agreement to implementation - Trust as the central enabling factor - Balancing ethics, incentives, and legal certainty - Key operational issues that require further work, especially regarding „Money IN“ (contributions), „Money OUT (disbursement)“, and Non-monetary benefit-sharing (NMBS) - Practical testing through pilots, i.e. moving from concepts to learning by doing Six initial pilots were developed, ranging from technical prototypes to full system validation. These could serve as basis for further discussions at COP17 and to generate evidence to further inform decisions at COP18. Overall, the dialogue confirmed that the foundations for the MLM and Cali Fund are in place, but their success will depend on practical implementation, trust-building, and stakeholder engagement. The report includes notes from all discussions, a documentation of group work results, and descriptions of the six pilots.